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001 per minute at 23°C with a 0
Readily accepts large DNAs for construction of large-insert genomic libraries
Tightly regulate expression from T7 promoters with HI-Control BL21(DE3) Chemically Competent Cells
200 ng of EcoR I-linearized pUC19 dsDNA and 1 µg of a 100-mer ssDNA oligo were mixed in 1X TA Buffer and incubated at 37°C for 20 min in the absence or presence of 10 units of Exonuclease I
This constitutively expressed gene
BES, monosodium salt size:25g 001 per minute at 23°C
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